Hamilton® PRP-C18 Reversed Phase HPLC Column, 5 µm
HPLC Column, 5 µm, 4.6 ID x 150 mm L, Stainless Steel, 100 Å Pore Size, PRP-C18 Stationary Phase, Reversed Phase Mode, PS-DVB Functionalized with C18 Packing Material, For Organic compounds
- Manufacturer Part No: 79676
- SECO Part No: HAM-79676
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Mobile phase pH is a powerful tool in methods development, particularly for separation of neutral forms of amines or other organic bases under alkaline conditions. Although some recent C18 columns boast stability in alkaline pH, all silica-based supports experience measurable degradation at pH > 6, where column life is still considerably shorter than if used under more favorable conditions. The PRP-C18, on the other hand, has genuine pH and chemical stability. The stationary phase stands up to prolonged exposure to concentrations as high as 1 M NaOH and H2SO4, with no measurable decrease in performance. Because the support does not strip, bleed, or dissolve at any pH, it therefore can be expected to perform reliably and reproducibly throughout the extended life of the column, regardless of mobile phase conditions.
| Diameter | : | 4.6 mm ID |
| Length | : | 150 mm |
| Material (ml/min) | : | Stainless Steel |
| Pore Size | : | 100 Å |
| Stationary Phase | : | PRP-C18 |
| Buffer Strength | : | 0.0 to 0.5 N |
| Compound Classification | : | Organics |
| Format | : | Column |
| Maximum Pressure | : | 5000 psi |
| Mobile Phase Limit | : | pH 1 to 13, 0 to 100% Aqueous, Organic Modifier |
| Mode | : | Reversed Phase |
| Packing Material | : | PS-DVB Functionalized with C18 |
| Particle Size | : | 5 µm |
| Restoration | : | Run a Gradient of 100% Water to 100% Acetonitrile |
| Temperature Limits | : | 5 to 85°C |
| USP Classification | : | USP L21 |
- Organic compounds: small molecules (< 2,000 mw), pharmaceuticals, steroids, halides, vitamins, amino acid analysis, herbicides
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