1. Choose columns based on functions:
Normal phase: solvent soluble solutions
Reverse phase: hydrocarbon soluble solutions
Ion-exchange: separate monosaccharides
Size-exclusion: separate proteins and carbohydrates
2. Diameter x Length vs Pressure:
Shorter and wider columns cut analysis time and require less pressure to move the mobile phase.
Longer and narrower columns provide higher resolution and more sensitive results. They require less samples for analysis but a higher pressure.
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