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Hamilton® PRP-3 Reversed Phase HPLC Column, 10 µm
HPLC Column, 10 µm, 4.1 ID x 150 mm L, Stainless Steel, 300 Å Pore Size, PRP-3 Stationary Phase, Reversed-Phase Mode, PSDVB Packing Material, For Protein Separations, Excellent Durability
- Manufacturer Part No: 79466
- SECO Part No: 17-1495-03
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Hamilton's PRP-3 is a polymeric reversed-phase HPLC column designed for the purification and isolation of proteins and peptides with very good recovery (> 90%). It is based off of the PRP-1 but utilizes a 300 Å pore size rather than the PRP-1's 100 Å pore size. The highly inert polymeric packing poly(styrene-divinylbenzene) enhances protein recovery because there are no silanol groups on the support to cause irreversible protein adsorption.
| Diameter | : | 4.1 mm ID |
| Length | : | 150 mm |
| Material (ml/min) | : | Stainless Steel |
| Pore Size | : | 300 Å |
| Stationary Phase | : | PRP-3 |
| Buffer Strength | : | 0.0 to 0.5 N |
| Compound Classification | : | Peptides/Proteins |
| Format | : | Column |
| Maximum Pressure | : | 5000 psi |
| Mobile Phase Limit | : | pH 1 to 13, 0 to 100% Aqueous, Organic Modifier |
| Mode | : | Reversed Phase |
| Packing Material | : | PSDVB |
| Particle Size | : | 10 µm |
| Restoration | : | Run a Gradient of 100% Water to 100% Acetonitrile |
| Temperature Limits | : | 5 to 85°C |
| USP Classification | : | USP L21 |
- Organic compounds: large molecules (> 2,000 mw), peptides, proteins, protein digests, protected and de-protected oligonucleotides, nucleic acids
- Polymeric 300 Å columns for protein separations
- pH stable from 0 to 14
- Better sample recovery than silica-based columns (there are no silanol groups)
- Excellent durability (the support can be cleaned with strong acid or base to remove any sample residue)
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