Hamilton® HC-75 Cation Exchange HPLC Column, 9 µm
HPLC Column, 9 µm, 7.8 ID x 305 mm L, Stainless Steel, Gel-type Pore Size, HC-75 Stationary Phase, Cation Exchange Mode, PSDVB/Sulfonic Acid Packing Material, For Cation, Ligand Exchange Separations of Carbohydrates
- Manufacturer Part No: 79436
- SECO Part No: 17-1135-10
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HC-40 and HC-75 columns separate compounds through size exclusion and ligand exchange. The 4% cross-linked HC-40 uses size exclusion as the primary mechanism of separation, while ligand exchange dominates in the more highly cross-linked HC-75. The different forms of the HC-75 (hydrogen, calcium and lead) each provide a unique selectivity for separating varying types of charged analytes based on electronegativity toward the counterion. The higher carbohydrate oligomers elute first while the smaller di- and monosaccharide's elute later.
| Diameter | : | 7.8 mm ID |
| Length | : | 305 mm |
| Material (ml/min) | : | Stainless Steel |
| Pore Size | : | Gel-type |
| Stationary Phase | : | HC-75 |
| Buffer Strength | : | Water |
| Compound Classification | : | Carbohydrates |
| Exchange Capacity | : | 5 meq/g |
| Exchange Capacity/Column | : | 32 meq/Column |
| Format | : | Column |
| Maximum Pressure | : | 400 psi |
| Mobile Phase Limit | : | 100% Water 0 to 40% Acetonitrile |
| Mode | : | Cation Exchange |
| Packing Material | : | PSDVB/Sulfonic Acid |
| Particle Size | : | 9 µm |
| Restoration | : | Flush with 1% Calcium Chloride at 0.1 ml/min Overnight |
| Temperature Limits | : | 25 to 90°C |
| USP Classification | : | USP L19 |
- Polymeric 7.5% cross-linked soft-gel columns for cation, ligand exchange separations of carbohydrates
- Compatible with UV and RI detectors
- Hydrogen form columns for USP L17 applications
- Calcium form columns for USP L19 applications
- Lead form columns for USP L34 applications
- Carbohydrates, sugar oligomers up to DP8. Mono and disaccharides, organic acids, sugars, and sugar alcohols
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